

You can use animated gifs in your signature but they will not animate unless you are using Outlook as part of a Microsoft 365 subscription.

Also, consider placing the image on the Internet and link to it instead. If it is larger than 10KB, then it will add to much overhead in relation to the message content itself.
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… insert an Internet image in my signature?.… prepare an image for signature usage?.This FAQ article is completely devoted to answer questions heard most often when using images in a signature. However, there are also some caveats to this practice. Adding an image to your Signature can your email look more professional or more personal.
